CLEC3B Knockout cell line (HEK293)

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CLEC3B Knockout cell line (HEK293) Order

CLEC3B Knockout cell line (HEK293)
Catalog#
YKO-XN37018
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CLEC3B Knockout cell line (HEK293)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
CLEC3B Knockout cell line (HEK293)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
CLEC3B
Gene ID
7123
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 2,Exon 1 (based on transcript CLEC3B-201)
Validation: PCR+Sanger Sequencing
CLEC3B Knockout cell line (HEK293) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CLEC3B gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HEK293 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CLEC3B gene knockout CLEC3B Knockout cell line (HEK293) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
CLEC3B Knockout cell line (HEK293)
CLEC3B Knockout cell line (HEK293)
Catalog#
YKO-XN37018
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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